Date of Award

4-1986

Degree Name

Master of Science

Department

Biological Sciences

First Advisor

Dr. Leonard C. Ginsberg

Second Advisor

Dr. Darwin Buthala

Third Advisor

Dr. Leonard Beuving

Access Setting

Masters Thesis-Open Access

Abstract

Yeast mannans are phosphorylated glycopeptides found in various species of yeast. Their similarity to the mannose-phosphate signal for intracellular lysosomal uptake led mannans to be considered as a possible vehicle for increasing cellular affinity for peptides. Insulin was used as a model peptide and was conjugated to mannans by two coupling methods. The conjugated mannan-insulins were compared to insulin on the basis of cellular binding and ability to induce typical insulin biological effects.

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