Date of Award

4-1985

Degree Name

Master of Science

Department

Biological Sciences

First Advisor

Dr. Darwin Buthala

Access Setting

Masters Thesis-Open Access

Abstract

This research defines the role and sequence of cell-mediated immune events in an in vivo virus infection. Pseudorabies virus infected CF1 mice lungs, were assessed by his to logical or immunohistochemical means. Using fluorescein isothiocyanate Tabled anti-T lymphocyte (ATG) and lissamine rhodamineB200 labeled anti-macrophage globulins (AMG), the number of lymphocytes and macrophages were determined. An early increase in T cells (four hours post-innoculation) was followed by an increase in macrophages (15 hours post-innoculation). Thereafter tissue levels of T cells and macrophages remained constant. Thymus dependent lymphocyte; failed to show increase macrophages clustering as pathology progressed. The first pathological changes in the bronchi and bronchiolar linings were the result of viral replication rather than the result of macrophage accumulation.

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