Date of Award
12-1985
Degree Name
Master of Science
Department
Biological Sciences
First Advisor
Dr. Gyula Ficsor
Second Advisor
Dr. Leonard Ginsberg
Access Setting
Masters Thesis-Open Access
Abstract
Groups of 5 male HA(ICR) mice were injected intraperitoneally with 60, 150, 300, or 600 mg/kg body weight of ethylmethanesulfonate or with a saline control. Each male was mated to two untreated females at two weeks after treatment and then again at five weeks after treatment. The treatment groups were labelled post-meiotic and pre-meiotic relative to the stage of spermatogenesis at time of treatment. Progeny were evaluated by observations of litter size, body weight, negative geotactic response, swimming patterns, limb use, water escape time and open field motor coordination activity. Body weight, geotactic response, limb use and open field motor coordination test results demonstrate that EMS causes a measurable genotoxic effect on both post- and pre-meiotic cells. This effect was qualitatively different between the two treatment groups using the geotactic response, limb use and open field motor coordination endpoints.
Recommended Citation
Goldner, Lee, "The Use of Motor Coordination Endpoints in the F1 Generation to Evaluate for Effects of Ethylmethanesulfonate on Pre- and Post-Meiotic Sperm Cells of Male Mice" (1985). Masters Theses. 1368.
https://scholarworks.wmich.edu/masters_theses/1368