Lee Goldner

Date of Award


Degree Name

Master of Science


Biological Sciences

First Advisor

Dr. Gyula Ficsor

Second Advisor

Dr. Leonard Ginsberg

Access Setting

Masters Thesis-Open Access


Groups of 5 male HA(ICR) mice were injected intraperitoneally with 60, 150, 300, or 600 mg/kg body weight of ethylmethanesulfonate or with a saline control. Each male was mated to two untreated females at two weeks after treatment and then again at five weeks after treatment. The treatment groups were labelled post-meiotic and pre-meiotic relative to the stage of spermatogenesis at time of treatment. Progeny were evaluated by observations of litter size, body weight, negative geotactic response, swimming patterns, limb use, water escape time and open field motor coordination activity. Body weight, geotactic response, limb use and open field motor coordination test results demonstrate that EMS causes a measurable genotoxic effect on both post- and pre-meiotic cells. This effect was qualitatively different between the two treatment groups using the geotactic response, limb use and open field motor coordination endpoints.