Date of Award
4-1984
Degree Name
Master of Science
Department
Biological Sciences
First Advisor
Dr. Robert C. Eisenberg
Second Advisor
Dr. Margaret Walker
Third Advisor
Dr. Michael E. McCarville
Access Setting
Masters Thesis-Open Access
Abstract
Mannitol-binding protein from Pseudomonas aeruginosa strain PAO has been purified 36-fold from magnesium chloride cold-shock supernatants. Sephadex G-100 gel filtration and carboxymethyl Sephadex ion exchange chromotography were utilized in the purification scheme. The position of the mannitol-binding protein band was identified on sodium dodeyl sulfate-polyacrylamide gels. The mannitol-binding protein has an apparent molecular weight of 45,000 as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
Recommended Citation
Plano, Gregory V., "Purification and Properties of a Mannitol-Binding Protein from Pseudomonas Aeruginosa" (1984). Masters Theses. 1391.
https://scholarworks.wmich.edu/masters_theses/1391