Author

Plano

Date of Award

4-1984

Degree Name

Master of Science

Department

Biological Sciences

First Advisor

Dr. Robert C. Eisenberg

Second Advisor

Dr. Margaret Walker

Third Advisor

Dr. Michael E. McCarville

Access Setting

Masters Thesis-Open Access

Abstract

Mannitol-binding protein from Pseudomonas aeruginosa strain PAO has been purified 36-fold from magnesium chloride cold-shock supernatants. Sephadex G-100 gel filtration and carboxymethyl Sephadex ion exchange chromotography were utilized in the purification scheme. The position of the mannitol-binding protein band was identified on sodium dodeyl sulfate-polyacrylamide gels. The mannitol-binding protein has an apparent molecular weight of 45,000 as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

Included in

Microbiology Commons

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