Date of Award


Degree Name

Master of Science


Biological Sciences

First Advisor

Dr. Robert C. Eisenberg

Second Advisor

Dr. Margaret Walker

Third Advisor

Dr. Michael E. McCarville

Access Setting

Masters Thesis-Open Access


Mannitol-binding protein from Pseudomonas aeruginosa strain PAO has been purified 36-fold from magnesium chloride cold-shock supernatants. Sephadex G-100 gel filtration and carboxymethyl Sephadex ion exchange chromotography were utilized in the purification scheme. The position of the mannitol-binding protein band was identified on sodium dodeyl sulfate-polyacrylamide gels. The mannitol-binding protein has an apparent molecular weight of 45,000 as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

Included in

Microbiology Commons