Date of Award
4-1983
Degree Name
Master of Science
Department
Biological Sciences
First Advisor
Dr. Leonard J. Beuving
Second Advisor
Dr. Cecil Mclntire
Access Setting
Masters Thesis-Open Access
Abstract
The streptozotocin-induced diabetic rat liver was analyzed for glucocorticoid receptor (GCR) content by a saturation and Scatchard analysis. A preliminary study was performed on the stabilization of the cytoplasmic GCR in a frozen cytosol preparation. The addition of 10% glycerol (V/V), 10 mM sodium molybdate, and 0.2 mg/ml trypsin inhibitor resulted in an approximate 20% sparing of GCR content upon freezing.
The hepatic GCR content of diabetic rats was significantly decreased from a control level of 0.1667 ± 0.0142 pmol/mg protein to 0.1094 ± 0.007 pmol/mg protein. Insulin replacement therapy to the diabetic rat dramatically increased the hepatic GCR content. The insulin treated diabetic rats had liver levels of GCR of 0.2602 ± 0.022 pmol/mg protein as compared to the diabetic value of 0.1094 ± 0.007 pmol/mg protein.
The results suggest insulin may have a possible role in the regulation of hepatic GCR content.
Recommended Citation
Yourick, Jeffrey John, "The Effects of Insulin on Hepatic Glucocorticoid Receptor Content in the Diabetic Rat" (1983). Masters Theses. 1642.
https://scholarworks.wmich.edu/masters_theses/1642