Date of Award


Degree Name

Master of Science


Biological Sciences

First Advisor

Dr. Leonard J. Beuving

Second Advisor

Dr. Cecil Mclntire

Access Setting

Masters Thesis-Open Access


The streptozotocin-induced diabetic rat liver was analyzed for glucocorticoid receptor (GCR) content by a saturation and Scatchard analysis. A preliminary study was performed on the stabilization of the cytoplasmic GCR in a frozen cytosol preparation. The addition of 10% glycerol (V/V), 10 mM sodium molybdate, and 0.2 mg/ml trypsin inhibitor resulted in an approximate 20% sparing of GCR content upon freezing.

The hepatic GCR content of diabetic rats was significantly decreased from a control level of 0.1667 ± 0.0142 pmol/mg protein to 0.1094 ± 0.007 pmol/mg protein. Insulin replacement therapy to the diabetic rat dramatically increased the hepatic GCR content. The insulin treated diabetic rats had liver levels of GCR of 0.2602 ± 0.022 pmol/mg protein as compared to the diabetic value of 0.1094 ± 0.007 pmol/mg protein.

The results suggest insulin may have a possible role in the regulation of hepatic GCR content.