The Detection of Behavioral Effects in the F(sub)1(/sub) Generation of Mice Treated During Spermatogenesis with Cyclophosphamide, Procarbazine or Mitomycin C

Author

Anthony Michael Podraza, Western Michigan University

Date of Award

4-1982

Degree Name

Master of Arts

Department

Psychology

First Advisor

Dr. Gyula Ficsor

Second Advisor

Dr. Malcolm Robertson

Third Advisor

Dr. Wayne Fuqua

Access Setting

Masters Thesis-Open Access

Abstract

Five CF₁ male mice were injected with 300 mg Procarbazine, 2 mg of Mitomycin C, 40 mg of Cyclophosphamide per kg body weight or saline control. Each male was mated to two untreated females 1-2 and 8-9 weeks after treatment, allowing for the evaluation of treatment upon spermatooza and spermatogonium, respectively. Offspring were evaluated through observations of reproduction. Dominant lethal effect was seen in the 8-9 week Cyclophosphamide, 1-2 and 8-9 week Procarbazine groups. Decreased weights were seen in all three treatment groups from the two mating times. In the water escape test, the 1-2 week Mitomycin C, 8-9 week Procarbazine, 1-2 and 8-9 week Cyclophosphamide groups took longer to escape than other groups. The surface righting test showed impaired motor reflex ability in the 1-2 week Mitomycin C and 8-9 week Cyclophosphamide groups. The 8-9 week Mitomycin C group demonstrated an impairment in limb use during the swim test. The developmental and behavioral impairments observed indicate a genetically transmissible effect.

Recommended Citation

Podraza, Anthony Michael, "The Detection of Behavioral Effects in the F(sub)1(/sub) Generation of Mice Treated During Spermatogenesis with Cyclophosphamide, Procarbazine or Mitomycin C" (1982). Masters Theses. 1711.
https://scholarworks.wmich.edu/masters_theses/1711