Date of Award


Degree Name

Master of Arts


Biological Sciences

First Advisor

Dr. Gyula Ficsor

Second Advisor

Dr. Walter Johnson

Third Advisor

Dr. Joseph Engemann

Access Setting

Masters Thesis-Open Access


Streptozocin-induced small colony variants of E. coli were characterized and found to have a genetic basis. Both E. coli and S. typhimurium were susceptible to the mutation causing small colonies. Small colony cell lines exhibited a greatly increased generation time and were shown to be non-motile, electron microscopy revealing a complete loss of flagella. The small colony phenotype was revertible by streptozotocin and nitrosoguanidine. The locus responsible for small colony phenotype in one strain of E. coli was found to map within a 19 minute segment of the chromosome between HfrH and HfrC.

Included in

Microbiology Commons