Date of Award
12-2009
Degree Name
Master of Science
Department
Biological Sciences
First Advisor
Dr. Maria E. Scott
Second Advisor
Dr. Silvia Rossbach
Third Advisor
Dr. Susan Stapleton
Access Setting
Masters Thesis-Campus Only
Abstract
To examine the secreted protein profile of Shiga toxin-producing Escherichia coli (STEC) we developed a method to enrich for low abundance secreted proteins. Rotary evaporation in combination with conventional spin column concentration achieved protein enrichment 10-fold greater than that acquired by the traditional trichloroacetic acid protein precipitation method. Analysis of the enriched secreted protein fraction led to the discovery of a protein designated as YghJ. Genetic homology analysis revealed that YghJ is a serine/threonine phosphatase-like protein. Serine/threonine phosphatases have not previously been discovered in Escherichia coli and are rare in bacteria. The probable serine/threonine phosphatase activity of YghJ suggests that the protein may play a role in the regulation of stress factors produced by E. coli during times of nutrient deprivation.
Recommended Citation
Trumble, Chad, "Enrichment of Low Abundance Proteins and Isolation of the Novel Serine/Threonine Phosphatase, YghJ, from Shiga Toxin-Producing Escherichia Coli O91:H21" (2009). Masters Theses. 302.
https://scholarworks.wmich.edu/masters_theses/302