Date of Award
4-2002
Degree Name
Master of Science
Department
Biological Sciences
First Advisor
Dr. Alexander Enyedi
Second Advisor
Dr. Silvia Rossbach
Third Advisor
Dr. Susan Stapleton
Access Setting
Masters Thesis-Open Access
Abstract
Inoculation of Arabidopsis thaliana with Pseudomonas syringae pv. tomato DC3000 AvrB (Pst) results in a series of biochemical changes, including an oxidative burst, accumulation of salicylic acid, (de)phosphorylation of proteins, localization of transcription factors to the nucleus and ultimately expression of pathogenesis-related genes. The redox status within plant cells, which is maintained by a cycle of antioxidants that are largely dependent on the reduced form of glutathione (GSH), because of its ability to donate electrons that are necessary to quench reactive oxygen species (ROS). This study focuses on the role of glutathione in the early defense responses of Arabidopsis to Pst. It is hypothesized that (1) a decrease in the overall [GSH] or (2) an increase in [GSSG] (a decrease in the [GSH]:[GSSG]), will affect salicylic acid accumulation and pathogenesis-related gene expression in Arabidopsis tissue inoculated with Pst.
Recommended Citation
McKenna, Allison Patrice, "The Role of Glutathione in the Defense Pathway Response of Arabidopsis Thaliana to the Pathogen Pseudomonas Syringae Pathovar Tomato DC3000 AvrB" (2002). Masters Theses. 4468.
https://scholarworks.wmich.edu/masters_theses/4468