Date of Award
12-1996
Degree Name
Master of Science
Department
Biological Sciences
First Advisor
Dr. Charles Mackenzie
Second Advisor
Dr. Cindy Hoorn
Third Advisor
Dr. Robert Eversole
Fourth Advisor
Dr. Leonard Beuving
Access Setting
Masters Thesis-Open Access
Abstract
The integrin α4β1 is expressed on a wide variety of peripheral leukocytes, and functions in recruitment of these cells to sites of inflammaiton. We infected august (AUG) rats with the helminth parasite Nippostrongylus brasiliensis and later used leukocytes from the peritoneum of the sensitized animals and antibodies against α4β1 integrin to localize that adhesion molecule on the cell surfaces after antigen challenge. We found that after antigen challenge, eosinophils, monocytes, and small lymphocytes, but not mast cells, expressed α4β1 integrin if the animals had been previously infected, but cells from uninfected animals did not show expression of this molecule. Our study describes a novel protocol for immunohistochemical analysis of rat eosinophils.
Recommended Citation
Phipps, John C., "Expression of α4β1 Integrin on Tissue Eosinophils in the August Rat" (1996). Masters Theses. 4522.
https://scholarworks.wmich.edu/masters_theses/4522