Author

Phipps

Date of Award

12-1996

Degree Name

Master of Science

Department

Biological Sciences

First Advisor

Dr. Charles Mackenzie

Second Advisor

Dr. Cindy Hoorn

Third Advisor

Dr. Robert Eversole

Fourth Advisor

Dr. Leonard Beuving

Access Setting

Masters Thesis-Open Access

Abstract

The integrin α4β1 is expressed on a wide variety of peripheral leukocytes, and functions in recruitment of these cells to sites of inflammaiton. We infected august (AUG) rats with the helminth parasite Nippostrongylus brasiliensis and later used leukocytes from the peritoneum of the sensitized animals and antibodies against α4β1 integrin to localize that adhesion molecule on the cell surfaces after antigen challenge. We found that after antigen challenge, eosinophils, monocytes, and small lymphocytes, but not mast cells, expressed α4β1 integrin if the animals had been previously infected, but cells from uninfected animals did not show expression of this molecule. Our study describes a novel protocol for immunohistochemical analysis of rat eosinophils.

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Biology Commons

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