Date of Award
8-1997
Degree Name
Master of Arts
Department
Chemistry
First Advisor
Dr. John L. Nappier
Second Advisor
Dr. Terry J. Gilbertson
Third Advisor
Dr. Scott A. Brown
Access Setting
Masters Thesis-Open Access
Abstract
Premafloxacin is a fluoroquinolone antibiotic being developed by Pharmacia & Upjohn for veterinary indications. Since the parent drug and possibly metabolites will be applied to soil through the deposition of animal excreta, many regulatory agencies will require environmental assessment testing. In order to determine if 14C-premafloxacin would biodegrade in the soil, loam soil was extracted with solvents and C18 solid phase columns were used to isolate the compound after three weeks of incubation. In addition to extracting the soil, production of 14CO2 was monitored by trapping the degradation by-product in 2 N NaOH. Liquid scintillation counting of the solvent extracts and trapping solution appeared to indicate a high binding affinity between 14C-premafloxacin and the soil. This affinity can reduce the bioavailability of the drug to microorganisms thus inhibiting aerobic biodegradation. Contact with loam soil will therefore immobilize the drug and naturally occurring environmental conditions involving weak acid, base and saline solutions will not dissociate the drug from the soil.
Recommended Citation
Staskiewicz, Steven Gregory, "Extraction of Premafloxacin Radiolabeled with Carbon Fourteen from Loam Soil" (1997). Masters Theses. 4794.
https://scholarworks.wmich.edu/masters_theses/4794