Quantitative Analysis of Steroids by ¹³C NMR Spectroscopy

Author

Pei Wang, Western Michigan University

Date of Award

4-1994

Degree Name

Master of Arts

Department

Chemistry

First Advisor

Dr. James A. Howell

Second Advisor

Dr. Ralph K. Steinhaus

Third Advisor

Dr. H. Dale Warren

Access Setting

Masters Thesis-Open Access

Abstract

Steroids are usually analyzed quantitatively by using HPLC with ultraviolet detection and are confirmed by FTIR, GC-MS. Quantitative ¹³C NMR method provide an alternative means to analyze steroids. Chromatographic separation and subsequent identification by match with a spectroscopic data base spectrum is not required for quantitative analysis of mixture components. Instead, equivalent information is obtained by creating subspectra from resonances with equivalent peak areas within a quantitative NMR spectrum. Techniques for eliminating Nuclear Overhauser Enhancement (NOE) effect is described.

Recommended Citation

Wang, Pei, "Quantitative Analysis of Steroids by ¹³C NMR Spectroscopy" (1994). Masters Theses. 5041.
https://scholarworks.wmich.edu/masters_theses/5041